A study on the mutation of P gene region including YMDD motif in hepatitis B virus.
- Author:
Bo FENG
1
;
Lai WEI
;
Ming CHEN
;
Xiu-hua LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Amino Acid Motifs; Base Sequence; Gene Products, pol; genetics; Hepatitis B virus; genetics; Humans; Male; Molecular Sequence Data; Mutation; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length
- From: Chinese Journal of Hepatology 2004;12(1):29-31
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the primary structure and heterogenenity of P gene region including YMDD motif in hepatitis B virus.
METHODSFrom serum samples collected from 4 patients who had never been treated with anti-viral drugs, DNA fragments of 1057bp long of P gene were amplified and cloned into pUC19. Twenty positive clones were chosen randomly from each sample. The YMDD motif mutation was detected by mismatched PCR-RFLP. Finally last ten positive clones of two samples were sequenced.
RESULTSNucleotide mutation rates among clones of Sample 1 and 2 were 0.3% - 1.1%, 0.4% - 1.7%, respectively. Among 80 clones, the variations from YMDD to YMGD were revealed in two clones.
CONCLUSIONThere are HBV quasispecies in the P gene region including YMDD motif of hepatitis B virus and a novel mutation of YMDD motif in the sera of patients without being therapied by anti-viral drugs.
