Reversal of multidrug resistance gene MDR1 and MRP of drug-resistant human hepatocellular carcinoma cells SMMC-7721/ADM with antisense phosphorothioate oligonucleotides.
- Author:
Hua-you LUO
1
;
Jia-yin YANG
;
Zi-ming LIU
;
Qi-yuan LIN
;
Lu-nan YAN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; drug therapy; genetics; Cell Line, Tumor; Daunorubicin; metabolism; pharmacology; Doxorubicin; pharmacology; Drug Resistance, Neoplasm; Genes, MDR; Humans; Liver Neoplasms; drug therapy; genetics; Multidrug Resistance-Associated Proteins; genetics; Oligonucleotides, Antisense; pharmacology; Rhodamine 123; metabolism
- From: Chinese Journal of Hepatology 2004;12(2):85-87
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the reversal effect of gene MDR1 and MRP with combinational antisense phosphorothioate oligonucleotide on Drug-resistant human hepatocellular carcinoma cells SMMC-7721/ADM.
METHODSSMMC-7721/ADM was transfected with synthetic antisense phosphorothioate oligonucleotides complementary to gene MDR1 and MRP mediated by Lipofectamine. Drug sensitivity was measured by MTT assay, Fluorescence intensity of cells was determined by flow cytometric analysis, RH123 and DNR retention was assayed by confocal scanning laser microscopy.
RESULTSASODN of MDR1+MRP increased the sensitivity of SMMC-7721/ADM to chemotherapeutic drug more significantly than that any of MDR1 and MRP did separately. But they did not enhance the inhibition expression of protein of p190 or p170.
CONCLUSIONDrug-resistance could be reversed significantly when antisense phosphorothioate oligonucleotide of MDR1+MRP were transfected into drug-resistant human hepatocellular carcinoma cells SMMC-7721/ADM together.
