The contrast study of Pre-S1 protein, HBV-DNA and HBeAg in diagnosing viral replication in patients with chronic hepatitis B.
- Author:
Qin LI
1
;
Gui-zhen SUN
;
Yu-xiang WEI
;
Yu-yuan MIN
;
Yu-long CONG
;
Xiao-juan OU
;
Bao-en WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; DNA, Viral; blood; Female; Hepatitis B Surface Antigens; blood; Hepatitis B e Antigens; blood; Hepatitis B virus; physiology; Hepatitis B, Chronic; virology; Humans; Male; Middle Aged; Protein Precursors; blood; Virus Replication
- From: Chinese Journal of Hepatology 2004;12(3):134-136
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the role of Pre-S1 protein in diagnosing viral replication in patients with chronic hepatitis B.
METHODS104 consecutive patients with chronic hepatitis B were included in the study, liver biopsy were performed in all patients. Serial serum samples were studied with the quantitative determination of HBV-DNA by a quantitative PCR assay, determination of Pre-S1 protein by ELISA.
RESULTSThe positive rates of HBV-DNA and Pre-S1 protein in patients with HBsAg HBeAg anti-HBc (+) both were 96.5%. The positive rates of HBV-DNA and Pre-S1 protein in patients with HBsAg anti-HBe anti-HBc (+) were 81.5%, 72.3%, respectively. The positive rates of HBV-DNA and Pre-S1 protein in patients with HBsAg anti-HBc (+) were 87.5%, 75.0%, respectively. It represented some patients with HBeAg (-) anti-HBe (+/-) still had viral replication. HBV-DNA>10(3) copy/ml as positive criteria for diagnosing viral replication, the positive rate of HBeAg, Pre-S1 were 31.5% (28/89), 80.9% (72/89) in patients with HBV-DNA>10(3) copy/ml, respectively. The concordance rates of HBeAg, Pre-S1 with HBV-DNA were 40.0% (42/104), 82.0% (85/104), respectively.
CONCLUSIONIt showed that Pre-S1 was more sensitive than HBeAg in diagnosing viral replication in patients with chronic hepatitis B.
