Genotyping of HBV DNA in Shenzhen and clinical manifestations.
- Author:
Jian-yi DAI
1
;
Zhi-lin SHI
;
Yong DAI
;
Hong DU
;
De-heng CHEN
;
Sha-yan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; DNA, Viral; analysis; Female; Genotype; Hepatitis B; virology; Hepatitis B virus; classification; genetics; Humans; Male; Middle Aged; Polymerase Chain Reaction
- From: Chinese Journal of Hepatology 2004;12(4):199-200
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect HBV DNA and its genotypes.
METHODSThe 6 isoforms of HBV DNA was detected out using of different probes by Polymerase Chain Reaction and Nucleic Acid hybridization.
RESULTSOf 150 HBV DNA positive patients who lived in Shenzhen, 50 samples (33%) are type B, 36 samples (24%) are type C, 13 samples (9%) are type D, 3 samples is type F, 1 sample is type A, 48 samples (31%) are mixed type. The ALT value was significantly higher in genotype B than in genotype C. HBe positivity were higher in genotype B than genotype C. HBeAg positivity were higher in genotype C than in genotype B. There are not obvious relations between genotype and age or sex.
CONCLUSIONIn the detected samples, the major genotype of HBV DNA is type B, several are type C, D. The type E haven't been found. There are some relations between all kinds of genotypes and the severity of hepatitis B.
