A three-dimension in vitro model for angiogenesis of hemangioma.
- Author:
Cheng-Hong JIANG
1
;
Fu-Lian ZHUANG
;
Ba-Rui HUANG
;
Zhi-Hui GUO
;
Yi-De XIE
;
Ya-Kuan ZHOU
;
Biao WANG
;
Wen-lie CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Endothelium, Vascular; Hemangioma; Humans; Models, Cardiovascular; Neovascularization, Pathologic
- From: Chinese Journal of Plastic Surgery 2005;21(5):364-367
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo create a three dimension (3D) in vitro model for angiogenesis of hemangioma.
METHODThe fragment of hemangioma specimen was embedded in fibrin gel to set up the three-dimension (3D) in vitro model for angiogenesis of hemangioma.
RESULTIn the model, microvessels grew out from the tissue fragments at the 2nd to 3rd day after culture, and at the 8th to 9th day a compact network of microvessels come into being, then tending to be stationary. The compact network around the tissue fragment was confirmed to be blood vessels by immunohistochemistry and electron microscopy.
CONCLUSIONThis model helps to study the mechanism of hemangioma angiogenesis and investigate the drugs of anti-angiogenesis.