OBJECTIVETo establish a new method to accurately measure the viability of fat for grafting, which can lay a foundation for the further study of fat transplantation.

METHODSWe centrifugated 5 groups of fat respectively at 1000 r/min, 2000 r/min, 3000 r/min, 4000 r/min and 5000 r/min, took out 12 samples from per group of centrifugated fat, 5 ml per sample, and put them into dishes to incubate with DMEM containing glucose for one hour. Simultaneously we set up 5 control dishes (only containing DMEM without fat) to incubate together with those experimental dishes. After incubating we measured the glucose consistency of DMEM in all dishes. The glucose consistency difference between the mean value of 5 control dishes and per experimental dish represented the glucose transportation quantity of per sample, in other words, the sample viability. We analyzed and compared the glucose transportation quantity of every group of samples with t test. The 5 groups of fat were also put in histopathologic examination and the amounts of intact adipocytes on per group of slides were compared with T test.

RESULTSThe glucose transportation quantities of group 1000 r/min, 2000 r/min, 3000 r/min, 4000 r/min, 5000 r/min gradually decreased in turn (P < 0.05). The histopathologic examination didn't show the difference among group 1000 r/min, 2000 r/min, 3000 r/min and 4000 r/min the 5000 r/min (P > 0.05), and only showed the amounts of intact adipocytes on slides of group 5000 r/min were significantly fewer than other four group (P < 0.05).

CONCLUSIONThe glucose transportation test can be used to accurately measure the viability of fat. The histopathologic examination can reveal fat viability roundly.