Effect of adeno-BMP7 transfection on osteogenesis of BMSCs.
- Author:
Lian ZHU
1
;
Fu-guo CHEN
;
Wei LIU
;
Lei CUI
;
Guang-dong ZHOU
;
De-li LIU
;
Yi-lin CAO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; Animals; Bone Marrow Cells; cytology; Bone Morphogenetic Protein 7; genetics; Bone Regeneration; Cell Culture Techniques; Cell Differentiation; Cells, Cultured; Goats; Mesenchymal Stromal Cells; cytology; Osteogenesis; Tissue Engineering; methods; Transfection
- From: Chinese Journal of Plastic Surgery 2006;22(1):59-62
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of adeno-BMP7 transfection on the biology of bone marrow stromal cells (BMSCs).
METHODSBone marrow was obtained from the goat. The BMSCs were isolated and cultured at the second passage. Once the cells attached and formed a monolayer with 70%-80% confluency, adeno-BMP7 (M.O.I. = 100) was added to the cells. After three days, calcium node was examined with staining; cell-coral compound was replanted subcutaneously.
RESULTSWith adeno-BMP7 transfection, BMP7 expression was detected with Western-blot; big calcium nodes were observed with staining. New bone formation was enhanced, which was evaluated by X-ray and histological examinations.
CONCLUSIONSBMSCs transfected with adeno-BMP7 show much stronger osteogenic ability.
