Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion.

Huan YU; Zi Yang; Xiaoyan Ding; Yanling Wang; Yiwei Han

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Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion.

Author: Huan YU ¹ ; Zi YANG ² ; Xiaoyan DING ¹ ; Yanling WANG ³ ; Yiwei HAN ¹
Author Information

1. Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.
2. Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China. Email: zi_yang@email.com.
3. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Publication Type:Journal Article
MeSH: Cell Line; Cells, Cultured; Fatty Acids, Nonesterified; pharmacology; Humans; Lipid Metabolism; Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase; metabolism; Trophoblasts; drug effects
From: Chinese Medical Journal 2014;127(19):3378-3382
CountryChina
Language:English
Abstract:
BACKGROUNDPreeclampsia (PE) is associated with abnormal fatty acid beta-oxidation (FAO), especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is unclear. The aim of this study was to explore the influence of various lengths fatty acids oxidation on invasiveness of trophoblasts.
METHODSPrimary human trophoblast cells and HTR8/SVneo cells were treated with fatty acids of various lengths. Morphological changes, lipid deposition and ultrastructure changes of trophoblast cells were detected. Cells invasiveness was determined by transwell insert. CPT1, CPT2 and long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) protein expression were analyzed. The correlation between intracellular lipid droplets deposition and cells invasiveness was evaluated.
RESULTSCells treated with long-chain fatty acids showed significant increased lipid droplets deposition, severe mitochondrial damage, decreased CPT2 and LCHAD protein expression (P < 0.05) but no significant difference in CPT1 protein expression (P > 0.05). Invasiveness of the trophoblast cells of the LC-FFA group significantly decreased (P < 0.05). Intracellular lipid droplets deposition was negatively correlated with invasivenss (R = -0.745, P < 0.05).
CONCLUSIONTrophoblast cells after stimulation with long chain fatty acids exist fatty acid oxidation disorders, and reduce the ability of trophoblastic invasion.