Detection of anti-HIV-1/2 and HIV nucleic acid in population of non-remunerated blood donors in Shenzhen.
- Author:
L Ou YANG
1
;
C HUANG
;
R CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Base Sequence; Blood Donors; China; epidemiology; DNA, Viral; analysis; genetics; Female; HIV; genetics; immunology; HIV Antibodies; blood; HIV Infections; epidemiology; immunology; Humans; Male; Middle Aged; Molecular Sequence Data; Polymerase Chain Reaction; RNA, Viral; blood; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid
- From: Chinese Journal of Experimental and Clinical Virology 2000;14(3):236-239
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the prevalent status of HIV-1 in population of non-remunerated blood donors in Shenzhen.
METHODS46,095 non-remunerated blood donors were tested for anti-HIV-1/2 by ELISA. The donors of anti-HIV positive were further detected for HIV DNA from peripheral blood mononuclear cells (PMBCs) by nested-PCR and HIV RNA from plasma by RT-PCR. The partial genome of env of 2 blood donors were sequenced.
RESULTSThe anti-HIV-1 was tested positive in 7 of 46 095 voluntary blood donors and the positive rate was 0.015%. In these 7 non-remunerated blood donors of anti-HIV-1 positive,7 were positive for HIV DNA in PMBCs and 5 positive for HIV RNA in plasma. The sequence analysis showed that 2 donors were infected by HIV-1 subtype E strains.
CONCLUSIONSThere exists HIV-1 subtype E infection in population of non-remunerated blood donors in Shenzhen. It is essential to detect and monitor strictly the population of non-remunerated blood donors.
