Analysis on HIV-1 genetics and threshold of drug resistance in Dehong prefecture of Yunnan province in 2013.

Yanling MA; Jibao Wang; Hui Xing; Min Chen; Shitang Yao; Huichao Chen; Jin Yang; Yanling LI; Song Duan; Email: Dhduansong@sinacom; Manhong Jia; Email: Jiamanhong@hotmailcom

Return

Analysis on HIV-1 genetics and threshold of drug resistance in Dehong prefecture of Yunnan province in 2013.

Author: Yanling MA ¹ ; Jibao WANG ² ; Hui XING ³ ; Min CHEN ¹ ; Shitang YAO ² ; Huichao CHEN ¹ ; Jin YANG ² ; Yanling LI ² ; Song DUAN ⁴ ; Email: DHDUANSONG@SINA.COM. ; Manhong JIA ⁵ ; Email: JIAMANHONG@HOTMAIL.COM.
Author Information

1. Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China.
2. Dehong Center for Disease Control and Prevention.
3. National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention.
4. Dehong Center for Disease Control and Prevention
5. Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China
Publication Type:Journal Article
MeSH: Anti-HIV Agents; pharmacology; therapeutic use; China; Drug Resistance, Viral; genetics; Genes, pol; Genotype; HIV Infections; drug therapy; virology; HIV-1; drug effects; genetics; Humans; Mutation; Polymerase Chain Reaction; Prevalence; Reverse Transcriptase Inhibitors
From:Chinese Journal of Epidemiology 2015;36(6):584-588
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the HIV-1 genotypes and transmitted drug resistance (TDR) in Dehong prefecture of Yunnan province in 2013.
METHODSReferring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS), 54 plasma samples of recently reported HIV-infected individuals, aged between 16 and 25 years, were collected in Dehong prefecture from January to August 2013. Genotyping of partial pol gene was performed by using reverse transcriptional PCR. HIV-1 genotype. Prevalent levels of HIV-1 drug resistance transmission were analyzed.
RESULTSForty-eight plasma samples were successfully sequenced and analyzed. Among them, 45.8% were Chinese and the rest 54.2% were all Burmese. Based on pol sequences, identified HIV genotypes included subtype C (41.7%), URF (31.3%), CRF01_AE (12.5%), CRF07_BC (10.4%), CRF08_BC (2.1%) and subtype B (2.1%), C subtype appeared dominated in Chinese while URF was dominated in Burmese. One drug resistant mutation to non-nucleoside reverse transcriptase inhibitors (NNRTIs) was detected in one sequence from Burmese. Based on the statistical method of HIVDR-TS, the prevalence of transmitted HIV-1 drug resistance was adjusted as < 5%.
CONCLUSIONDiverse HIV-1 genotypes were found in this study, and the current HIV-1 drug resistant strains transmission was catalogued as at low prevalence level, in Dehong. To prevent the increase of the prevalence of transmitted HIV-1 drug resistance, standard treatment and scientific management for people living with HIV/AIDS should be strictly followed. Meanwhile, relevant surveillance, including drug resistance surveillance should also be performed among cross-border migrant population.