Expression level changes of inhibitor of differentiation 1 during ATRA-induced acute promyelocytic leukemia cells differentiation.
- Author:
Pei-zheng ZHENG
1
;
Chun-jun ZHAO
;
Hui-yong FAN
;
Yu-long CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; therapeutic use; Cell Differentiation; Humans; Inhibitor of Differentiation Protein 1; genetics; metabolism; Leukemia, Promyelocytic, Acute; drug therapy; metabolism; pathology; Oligonucleotide Array Sequence Analysis; Tretinoin; therapeutic use
- From: Chinese Journal of Hematology 2008;29(6):366-369
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of inhibitor of differentiation 1 (ID1) in ATRA-induced acute promyelocytic leukemia (APL) cells differentiation.
METHODSThe expression of ID1 was detected by cDNA microarray, cycloheximide inhibition test, real-time RT-PCR and western blot.
RESULTSThe expression of ID1 gene was up-regulated in ATRA-induced NB4 cells and APL cells from two patients and was independent on other proteins synthesis. ID1 expression level reached the peak at 2 h in NB4 cells induced by ATRA, its relative expression level was (359.4 +/- 48.7)-fold greater than control. ID1 expression level reached the peak at 2 h in bone marrow cells from APL patents treated with ATRA, and its level detected 3 times in one of the patient was (311.1 +/- 48.7) fold of control. The expression of ID1 protein was not up-regulated in ATRA resistant NB4-R2 cells after ATRA treatment.
CONCLUSIONID1 may be involved in ATRA-induced granulocytic differentiation as an ATRA-targeted gene.
