Influence of gene transduction mediated by lentivirus vector on gene expression of human CD34+ cord blood cells.
- Author:
Yuan-song BAI
1
;
Yu-bing CHEN
;
Zhang-zhen SHI
;
Zhen-xia LU
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; Cells, Cultured; Fetal Blood; cytology; Gene Expression Profiling; Genetic Vectors; Humans; Lentivirus; genetics; Oligonucleotide Array Sequence Analysis; Transduction, Genetic; Transfection
- From: Chinese Journal of Hematology 2008;29(6):393-396
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of gene transduction mediated by lentivirus vector on human CD34+ cord blood cell (CBCs) gene expression.
METHODSCD34+ cells were isolated and transduced with the third-generation self-inactivating ( SIN) lentiviral vector carrying green fluorescent protein (GFP). The total RNA from transduced cells was extracted and the differences of genotypes between the transduced and non-transduced CD34+ cells were determined with cDNA microarray analysis.
RESULTSIn 23000 genes two were upregulated and six downregulated. These changes were not confirmed by semi-quantitative RT-PCR method.
CONCLUSIONSLentiviral vector used in this study do not influence significantly on the gene expression of CD34+ CBCs, and the vector system may be a useful and safe one in clinical gene therapy.
