Stem cells modified by brain-derived neurotrophic factor to promote stem cells differentiation into neurons and enhance neuromotor function after brain injury.
- Author:
Sai ZHANG
1
;
Xiao-zhi LIU
;
Zhen-lin LIU
;
Yan-min WANG
;
Qun-liang HU
;
Tie-zhu MA
;
Shi-zhong SUN
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Apoptosis; Brain Injuries; physiopathology; therapy; Brain-Derived Neurotrophic Factor; analysis; genetics; Cell Differentiation; Glial Fibrillary Acidic Protein; Humans; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells; cytology; Mice; Nerve Tissue Proteins; analysis; Neurons; cytology; Phosphopyruvate Hydratase; analysis; Transfection
- From: Chinese Journal of Traumatology 2009;12(4):195-199
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo promote stem cells differentiation into neurons and enhance neuromotor function after brain injury through brain-derived neurotrophic factor (BDNF) induction.
METHODSRecombinant adenovirus vector was applied to the transfection of BDNF into human-derived umbilical cord mesenchymal stem cells (UCMSCs). Enzyme linked immunosorbent assay (ELISA) was used to determine the secretion phase of BDNF. The brain injury model of athymic mice induced by hydraulic pressure percussion was established for transplantation of stem cells into the edge of injury site. Nerve function scores were obtained, and the expression level of transfected and non-transfected BDNF, proportion of neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP), and the number of apoptosis cells were compared respectively.
RESULTSThe BDNF expression achieved its stabilization at a high level 72 hours after gene transfection. The mouse obtained a better score of nerve function, and the proportion of the NSE-positive cells increased significantly (P<0.05), but GFAP-positive cells decreased in BDNF-UCMSCs group compared with the other two groups (P<0.05). At the site of high expression of BDNF, the number of apoptosis cells decreased markedly.
CONCLUSIONBDNF gene can promote the differentiation of the stem cells into neurons rather than glial cells, and enhance neuromotor function after brain injury.
