Study the effects of flavonoids of Rhizoma Drynariae on gene level of rats without ovaries by technology of cDNA array.
- Author:
Yan-Ming XIE
1
;
Lin-Lin QIN
;
Jin-Biao CHEN
;
Tian-Hong CUI
;
Wen-Jun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Flavonoids; isolation & purification; pharmacology; Gene Expression Profiling; Gene Expression Regulation; drug effects; Oligonucleotide Array Sequence Analysis; Ovariectomy; Plants, Medicinal; chemistry; Polypodiaceae; chemistry; Random Allocation; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2005;30(14):1092-1095
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of flavonoids of Rhizoma Drynariae on gene level of rats without ovaries (OP) by technology of cDNA array.
METHODThe models of rats without ovaries were made After. 24 weeks of treatment, put them to death and sampled 6 ml blood from the abdominal aorta and 4cm spinal cord. Take out the total RNA, then separate the mRNA to check.
RESULTThere is 70 genes difference between the blank control group and model group, 9 genes difference between the blank control group and flavonoids of Rhizoma Drynariae group. The genes over expression of OP rats models regain normal after the models were fed on flavonoids of Rhizoma Drynariae. There are no differences in the spinal cords genes in the cDNA array analysis of this trail.
CONCLUSIONThe genes over expression of OP rats models regain normal after the models were fed on flavonoids of Rhizoma Drynariae for 6 months. The result shows that the flavonoids of Rhizoma Drynariae does have certain effects on the gene expression of rats without ovaries.
