Qualitative and quantitative methods of betulonic acid in fruits of Liquidambar formosana.
- Author:
Qi-Wei ZHANG
1
;
Yong-Xin ZHANG
;
Yu-Ru SUN
;
Dong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Thin Layer; methods; Fruit; chemistry; Liquidambar; chemistry; Oleanolic Acid; analogs & derivatives; analysis; isolation & purification; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2005;30(15):1168-1170
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop the identification and assay methods for betulonic acid in fruits of Liquidambar formosana.
METHODTLC method was used for identification with silica gel G plate and petroleum ether(60-90 degrees C)-acetone (17:3) as a developing solvent. Betulonic acid in ethanol extract was separated on the ODS column with methanol-water-glacial acetic acid (87:13:0.1) as mobile phase. Flow-rate was 1.0 mL x min(-1), and column temperature 35 degrees C. ELSD drift-tube temperature was 82 degrees C, and gas flow 1.25 L x min(-1).
RESULTThe qualitative method is repeatable. Betulonic acid in ethanol extracts is well separated, relationship of logarithms of injection amount and peak area is linear (r = 0.9997) within the range of 0.65-3.25 microg. The average recovery is 98.0% and RSD of repeatability is 2.5%. 11 crude drugs purchased from different areas in the country were identified and quantified with the methods.
CONCLUSIONThe methods and data could be used for quality control of fruits of L. formosana.
