The effect of Siwu Tang on EPO and G-CSF gene expression in bone marrow of irradiated blood deficiency mice.
- Author:
Ping GUO
1
;
Qian-De LIANG
;
Jian-Jiang HU
;
Ji-Feng WANG
;
Sheng-Qi WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow; metabolism; Bone Marrow Cells; cytology; Cell Count; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Erythroid Precursor Cells; cytology; Erythropoietin; biosynthesis; genetics; Female; Granulocyte Colony-Stimulating Factor; biosynthesis; genetics; Leukocyte Count; Medicine, Chinese Traditional; Mice; Mice, Inbred C57BL; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Whole-Body Irradiation
- From: China Journal of Chinese Materia Medica 2005;30(15):1173-1176
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the blood enriching function of Siwu Tang and its effect on Epo and G-CSF gene expressions in bone marrow of blood deficiency mice, and thus provide the basis for understanding the molecular mechanism of blood enriching function of Siwu Tang.
METHODThe animal model of blood deficiency were established in the mice by using 3.5 Gy60Co gamma-ray. Peripheral blood cells were analyzed and CFU-GM, BFU-E, CFU-E and CFU-mix were counted in bone marrow colony cultured. Both Epo and G-CSF gene expressions in bone marrow were measured with RT-PCR.
RESULTSiwu Tang significantly increased the number of peripheral blood cells and the amount of CFU-GM, BFU-E, CFU-E and CFU-mix in bone marrow and enhanced Epo and G-CSF gene expression in bone marrow in the mice with blood deficiency.
CONCLUSIONThe promotion of Epo and G-CSF gene expressions in bone marrow may be one of the mechanisms underlying the blood enriching function of Siwu Tang decoction.
