The simultaneous determination of laetrile, paeoniflorin and paeonol in Jingzhi Guizhi Fuling capsule by HPLC.
- Author:
Zheng-gen LIAO
1
;
Ya LING
;
Yan ZHONG
;
Qi-neng PING
Author Information
- Publication Type:Journal Article
- MeSH: Acetophenones; analysis; Amygdalin; analysis; Benzoates; analysis; Bridged-Ring Compounds; analysis; Capsules; Chromatography, High Pressure Liquid; methods; Cinnamomum; chemistry; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Glucosides; analysis; Monoterpenes; Paeonia; chemistry; Plants, Medicinal; chemistry; Polyporales; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2005;30(16):1252-1254
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC method for the simultaneous determination of three major bioactive components in Jingzhi Guizhi Fuling capsules namely laetrile, paeoniflorin and paeonol.
METHODA LiChrospher C18 column (4.6 mm x 250 mm, 5 microm) was used. The chromatography was carried out with a stepwise gradient programming. The mobile phase was acetonitrile-water (containing 0.1% phosphorous acid) and the flow rate was 1.0 mL x min.
RESULTThe linear range of laetrile was 12.87-102.94 micron x mL(-1), r = 0.999 9, paeoniflorin 24.84 - 198.7 microg x mL(-1), r = 0.9999 and paeonol 12.57-100.56 microg x mL(-1), r = 0.999 9. The method is accurate with variation less than 1.5 % and recovery more than 95 %.
CONCLUSIONThe method was successfully applied to analyze three major bioactive components in Jingzhi Guizhi Fuling capsules.
