Determination of two main components in bark of Paeonia suffruticosa by HPLC.
- Author:
Yuan-zi TIAN
1
;
Yan WANG
;
Jian-zhen LIU
;
Bei-bei YANG
;
Jian-gong SHI
;
Mu-zou WANG
Author Information
- Publication Type:Journal Article
- MeSH: Acetophenones; analysis; Chromatography, High Pressure Liquid; methods; Gallic Acid; analysis; Paeonia; chemistry; Plant Bark; chemistry; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2005;30(16):1265-1268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa.
METHODThe reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm.
RESULTThe assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration.
CONCLUSIONThe method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.
