Experimental study of saponins from Tribulus terrestris on renal carcinoma cell line.
- Author:
Huang-jian YANG
1
;
Wei-jing QU
;
Bin SUN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; isolation & purification; pharmacology; Apoptosis; drug effects; Carcinoma, Renal Cell; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Cyclin D1; metabolism; Drugs, Chinese Herbal; isolation & purification; pharmacology; Humans; Kidney Neoplasms; metabolism; pathology; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-bcl-2; metabolism; S Phase; drug effects; Saponins; isolation & purification; pharmacology; Tribulus; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(16):1271-1274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of saponins from Tribulus terrestris (STT) on the renal carcinoma cell (786-0) in vitro, and inhibitory mechanisms.
METHODEffects of SIT on the cytotoxicity, morphological changes of apoptosis, cell cycle and expression of Bcl-2 protein in the 786-0 were tested respectively by MTT method, Wright and acridine orange stain assay, as well as flow cytometry (FCM).
RESULTAfter the 786-0 was treated by STY, it was shown that: 1) A significant cytotoxic effect was observed by MTT assay; 2) Apoptosis-induced was viewed by Wright and acridine orange stain assay; 3) The distribution of 786-0 on S phase was increased; 4.) The expression of Bcl-2 protein and cyclin D1 was decreased.
CONCLUSIONSTT can significantly inhibit the growth of 786-0 in vitro, partially, by apoptosis.
