The effect of tetrandrine on the expression of the P170, LRP and TOPO II in S180's tumor cell induced by chemotherapy in the mice with acquired multi-drug resistance.
- Author:
Gui-hai LI
1
;
Ming-xia LIU
;
Fu-jun SUN
;
Ning WANG
;
Ge-ping YIN
;
Xiao-jing LI
;
Wei-feng LI
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; metabolism; Alkaloids; pharmacology; Animals; Benzylisoquinolines; pharmacology; DNA Topoisomerases, Type II; metabolism; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Drugs, Chinese Herbal; pharmacology; Gene Expression Regulation, Neoplastic; Genes, MDR; Mice; Random Allocation; Sarcoma 180; enzymology; metabolism; pathology; Vault Ribonucleoprotein Particles; metabolism
- From: China Journal of Chinese Materia Medica 2005;30(16):1280-1282
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of tetrandrine on the P170 production expressed by multi-drug resistance gene, lung resistant protein (LRP), and topoisomeras II and elucidate the underlying molecular mechanism.
METHODCellular model of multi-drug resistance was established in S180 tumor cell by means of the scheme of PFC chemotherapy at the dosage lower than that with curative effect. P170, LRP and TOPO II were measured by flow cytometry after the mouse model was treated with tetrandrine for 4 weeks.
RESULTtetrandrine obviously reduced the enhancement of express of P170, LRP and the activity of TOPO II in the tumor cells with multi-drug resistance induced by chemotherapy.
CONCLUSIONTetrandrine significantly inhibits the multi-drug resistance of tumor cells induced by chemotherapy via diminishing both the expression of multi-drug resistance gene and the activity of topoisomeras II.
