The qualitative analysis and quantitative analysis of purification of salvianolic acids by macroreticular resin.
- Author:
Xin-sheng FANG
1
;
Xiao-mei TAN
Author Information
- Publication Type:Journal Article
- MeSH: Benzofurans; analysis; isolation & purification; Caffeic Acids; analysis; isolation & purification; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Ion Exchange Resins; Lactates; analysis; isolation & purification; Salvia miltiorrhiza; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(17):1331-1334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo purify salvianolic acids by macroreticular resin,then mensurate the contents of salvianolic acids and analyse the chromatogram with HPLC.
METHODMake salvianolic acids with macroreticular resin; mensurate the content of Salvianolic acids with UV spestrophotometry: the control compound is protocaechuic aldehyde, and the wavelength is 281 nm. Analysis the chromatogram with HPLC, and compare the chromatogram in different technics: zorbax ODS column (4.6 mm x 250 mm, 5 microm), mobilephase: 1% aceticacid-water and methanol in different proportions, the wavelength is 281 nm.
RESULTThe contents of salvianolic acids is 53.8%; HPLC chromatogram indicate that the method is reasonable to make salvianolic acids.
CONCLUSIONDetermination of contents and HPLC chromatogram can control the quality of Salvianolic acids more accurately.
