Study on the extraction recovery of ginsenoside Re in plasma by different solid-phase cartridges.
- Author:
Xiang-Jun MAO
1
;
Chen-Feng ZHANG
;
Di SUN
;
Long-Sheng SHENG
;
Guang-Ji WANG
;
Wen-Ying LIU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Ginsenosides; blood; isolation & purification; Humans; Panax; chemistry; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(19):1516-1518
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the solid-phase extraction method by comparison of the extraction recovery of ginsenoside Re plasma samples.
METHODAfter extracted by different solid-phase cartridges with water, acetonitrile, and different content methanol elution, the plasma samples were analyzed on an Zorbax SB-C18 column with acetonitrile-water gradient elution. From the recovery achieved, the best solid phase cartridge was found.
RESULTThis method consists of using 40% methanol as the wash solvent, and 80% methanol for the elution. Among the three kinds of solid-phase being tested, Waters Oasis HLB cartridge was found to be the best one.
CONCLUSIONThe average extraction recovery of the Waters Oasis HLB cartridges was between 103%-113%, it can be used in the analysis of ginsenoside Re in plasma samples.
