Experimental study of chronic renal tubular-interstitial injury induced by Radix Aristolochiae Fangchi Extract in rats.
- Author:
Gui-You DU
1
;
Su-Juan ZHOU
;
Yong ZHAO
;
Hai-Feng CU
;
Xiu-Rong WANG
;
Li LI
;
Yong-Qing XIAO
;
Chun-Yun CAO
;
Zi-Lun WU
;
Shuang-Rong GAO
;
Rong HE
;
Lian-Qiang HUI
;
Bao-Yan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aristolochia; chemistry; toxicity; Aristolochic Acids; isolation & purification; toxicity; Blood Urea Nitrogen; Body Weight; drug effects; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; administration & dosage; isolation & purification; toxicity; Female; Fibrosis; blood; chemically induced; pathology; Kidney Tubules; pathology; Male; Nephritis, Interstitial; blood; chemically induced; pathology; Plant Roots; chemistry; toxicity; Plants, Medicinal; chemistry; toxicity; Proteinuria; chemically induced; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2005;30(19):1527-1532
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEFollowing the former report, we continue to observe the chronic renal tubular-interstitial injury induced by Radix Aristolochiae Fangchi Extract(RAFE) in rats in order to understand whether RAFE in different doses causes the renal tubular-interstitial injury or not.
METHODRAFE at the dose of 25.0 mg x kg(-1) x d(-1), 120.0 mg kg(-1) x d(-1) and 200.0 mg x kg(-1) x d(-1) and aristolochic acid (AA, 10.0 mg x kg(-1) d(-1)) was interruptedly administrated by gastric tube for 22 w and 4 w durg withdrawal. Blood, urine and kidney were taken out respectively in 17 w, 22 w and 26 w to measure the indexes of renal function. The morphology of kidney was observed, and Masson staining of kidney were made respectively to compare RAFE groups with AA group.
RESULTPathological changes of renal tissue forms were as follows: All RAFE groups and AA group could develop the pathological process of renal tubular injury-chronic renal interstitial fibrosis. The pathologic changes of RAFE were similar with AA.
CONCLUSIONRAFE at all doses administrated interruptedly by gastric tube above 13 w caused chronic renal tubulo-interstitium fibrosis. The renal injury in functions and tissue forms in rats were similar with AA closely. The results showed that AA was the main toxic composition of RAFE.
