Determination of catechin and its analogues in Spatholobus suberectus by RP-HPLC.
- Author:
Chao LIU
1
;
Lin MA
;
Ruo-Yun CHEN
;
Ping LIU
Author Information
- Publication Type:Journal Article
- MeSH: Catechin; analogs & derivatives; chemistry; isolation & purification; Chromatography, High Pressure Liquid; methods; Fabaceae; chemistry; Molecular Structure; Plant Stems; chemistry; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2005;30(18):1433-1435
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for determination of catechin and its analogues in Spatholobus suberectus.
METHODGallocatechin, catechin and epicatechin are used as reference substances. Chromatographic assay is performed on a C18 colum. The mobile phase is composed of methanol and acetic acid solution with gradient elution. Velocity of flow is 0.8 mL x min(-1) and the detection wavelength is 270 nm.
RESULTThe calibration curves of gallocatechin, catechin and epicatechin are linear within the range of 0.397 microg to 1.986 microg, 0.404 microg to 2.019 microg and 0.405 microg to 2.024 microg. The recoveries of them are 97.2%, 100.8% and 98.8%, and the RSD are 1.7%, 2.4%, 2.4% (n = 5).
CONCLUSIONThe method is simple, rapid and suitable for the determination of catechin and its analogues in S. suberectus.
