Studies on the changes of 5-hydroxymethyl furfuraldehyde content in Radix Rehmanniae steamed for different time.
- Author:
Jun LI
1
;
Li-Ping ZHANG
;
Wei LIU
;
Zhen-Ling ZHANG
;
Li-Juan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Furaldehyde; analogs & derivatives; analysis; Hot Temperature; Plants, Medicinal; chemistry; Quality Control; Rehmannia; chemistry; Rhizome; chemistry; Technology, Pharmaceutical; methods; Time Factors
- From: China Journal of Chinese Materia Medica 2005;30(18):1438-1440
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo find the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Radix Rehmanniae steamed for different time.
METHODHPLC method was used, with C18 column (6 mm x 200 mm, 5 microm), a mixture of methanol-water (15: 85) as the mobile phase. The flow rate was 1.0 mL x min(-1). The UV detection was setat 284 nm and the column temperature was set at 24 degrees C.
RESULTThe linear range was from 0.01 to 0.08 microg, r = 0.999 9 (n = 5). The average recovery was 100.2% and RSD was 2.7% (n = 5).
CONCLUSIONThe 5-HMF content increases as the processing time prolongs in a certain time rang.
