Overexpression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes.

Liang Bai; Yali Zhang; Chen Xie; Rong Wang; Sihai Zhao; Yuzhi Jia; Enqi Liu

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Overexpression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes.

Author: Liang BAI ^{1
,

2} ; Yali ZHANG ¹ ; Chen XIE ¹ ; Rong WANG ¹ ; Sihai ZHAO ¹ ; Yuzhi JIA ³ ; Enqi LIU ¹ ;
Author Information

1. Research Institute of Atherosclerotic Disease, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China
2. Laboratory Animal Center, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
3. Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
Publication Type:Journal Article
MeSH: Adipocytes; metabolism; Adipogenesis; Animals; Cells, Cultured; Fatty Liver; metabolism; pathology; Genetic Vectors; Hepatocytes; metabolism; pathology; Mice; Mice, Inbred C57BL; PPAR gamma; metabolism; Transfection
From: Journal of Zhejiang University. Medical sciences 2016;45(1):68-74
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effects of PPARγ overexpression on steatosis in mouse primary hepatocytes.
METHODSPrimary hepatocytes isolated from C57BL/6J mice were infected with either Ad/LacZ or Ad/PPARγ for 48 h. Steatosis of the primary hepatocytes was checked by Oil Red O staining. The mRNA and protein expression of adipocyte-specific genes PPARγ, aP2 and CideA were analyzed by using RT Real-time PCR and Western Blot.
RESULTSPrimary hepatocytes were small and even. Hepatocyte nuclei were round with dispersed chromatin and prominent nucleoli. Accumulated lipid droplets were observed in Ad/PPARγ-infected hepatocytes, but in Ad/LacZ-infected hepatocytes. Moreover, compared with Ad/LacZ-infected hepatocytes, the mRNA expression of PPARγ, aP2, FGF21 and CideA in Ad/PPARγ-infected hepatocytes were significantly induced, the protein expression of PPARγ and its target aP2 strongly increased.
CONCLUSIONover expression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes.