Fabrication of bioactive tissue engineering scaffold for reconstructing calcified cartilage layer based on three-dimension printing technique.

Xinning YU; Jinghua Fang; Jianyang Luo; Xianyan Yang; Dongshuang HE; Zhongru Gou; Xuesong Dai

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Fabrication of bioactive tissue engineering scaffold for reconstructing calcified cartilage layer based on three-dimension printing technique.

Author: Xinning YU ¹ ; Jinghua FANG ¹ ; Jianyang LUO ¹ ; Xianyan YANG ² ; Dongshuang HE ² ; Zhongru GOU ³ ; Xuesong DAI ⁴
Author Information

1. Department of Orthopedic Surgery, Orthopedics Research Institute of Zhejiang University, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
2. Zhejiang-California International Nanosystems Institute, Hangzhou 310058, China.
3. Zhejiang-California International Nanosystems Institute, Hangzhou 310058, China. zhrgou@zju.edu.cn.
4. Department of Orthopedic Surgery, Orthopedics Research Institute of Zhejiang University, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China. daixshz@163.com.
Publication Type:Journal Article
MeSH: Bioprinting; Cartilage; growth & development; Materials Testing; Porosity; Printing, Three-Dimensional; Tissue Engineering; methods; Tissue Scaffolds; chemistry
From: Journal of Zhejiang University. Medical sciences 2016;45(2):126-131
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo fabricate organic-inorganic composite tissue engineering scaffolds for reconstructing calcified cartilage layer based on three-dimensional (3D) printing technique.
METHODSThe scaffolds were developed by 3D-printing technique with highly bioactive calcium-magnesium silicate ultrafine particles of 1%, 3% and 5% of mass fraction, in which the organic phases were composed of type I collagen and sodium hyaluronate. The 3D-printed scaffolds were then crosslinked and solidified by alginate and CaCl₂ aerosol. The pore size and distribution of inorganic phase were observed with scanning electron microscope (SEM); the mechanical properties were tested with universal material testing machine, and the porosity of scaffolds was also measured.
RESULTSPore size was approximately (212.3 ± 34.2) μm with a porosity of (48.3 ± 5.9)%, the compressive modulus of the scaffolds was (7.2 ± 1.2) MPa, which was irrelevant to the percentage changes of calcium-magnesium silicate, the compressive modulus was between that of cartilage and subchondral bone.
CONCLUSIONThe porous scaffolds for calcified cartilage layer have been successfully fabricated, which would be used for multi-layered composite scaffolds in osteochondral injury.