Expression of miR-let-7e-3p in cervical intraepithelial neoplasm and cervix carcinoma and its clinical significance.
- Author:
Xiaojing CHEN
1
;
Junfen XU
1
;
Jing YE
1
;
Xiaodong CHENG
1
;
Xing XIE
1
;
Weiguo LYU
2
Author Information
1. Central Laboratory, Women's Hospital, Zhejiang University School of Medicine, Key Laboratory of Women's Reproductive Health of Zhejiang Province, Hangzhou 310006, China.
2. Central Laboratory, Women's Hospital, Zhejiang University School of Medicine, Key Laboratory of Women's Reproductive Health of Zhejiang Province, Hangzhou 310006, China. lbwg@zju.edu.cn.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
genetics;
Carcinoma;
chemistry;
genetics;
Cell Cycle;
drug effects;
genetics;
Cell Line, Tumor;
chemistry;
drug effects;
physiology;
Cell Movement;
drug effects;
genetics;
Cell Proliferation;
drug effects;
genetics;
Cervical Intraepithelial Neoplasia;
chemistry;
genetics;
physiopathology;
Down-Regulation;
physiology;
Female;
Humans;
MicroRNAs;
analysis;
pharmacology;
Neoplasm Invasiveness;
genetics;
physiopathology;
Neoplastic Processes;
Real-Time Polymerase Chain Reaction;
Transfection;
Uterine Cervical Neoplasms;
chemistry;
genetics;
physiopathology
- From:
Journal of Zhejiang University. Medical sciences
2016;45(4):342-348
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the expression of microRNA (miRNA, miR) let-7e-3p in different cervical lesions and its clinical significance.The expression of miR-let-7e-3p in the tissues of normal cervix (=26), high-grade squamous intraepithelial lesion (HSIL) (=37), and cervix carcinoma (=101) were detected by reverse transcription and quantitative polymerase chain reaction (RT-qPCR). The correlation of miR-let-7e-3p expression with the clinicopathological parameters of patients with cervical cancer was analyzed. miR-let-7e-3p mimic was transfected into cervical carcinoma Siha cells. The cell cycle and apoptosis were determined by flow cytometry; cell proliferation was determined by CCK-8 kit; and the migration and invasion of cells were determined by Transwell assay.The relative expression levels of miR-let-7e-3p in normal cervix, HSIL, and cervical carcinoma were 1.45±0.24, 0.79±0.05 and 0.46±0.04, respectively (all<0.05). After transfection with miR-let-7e-3p mimic, the S-phase fraction and apoptosis rate of Siha cells were increased significantly compared with control group[(29.76±6.6)% vs (13.38±1.3)%,<0.05; (5.98±1.38)% vs (3.53±0.79)%,<0.05, respectively]. OD of transfected Siha cells at 48, 72 and 96 h were 0.57±0.11,0.65±0.04 and 0.84±0.14, which were significantly lower than those of untransfected Siha cells (0.74±0.05, 0.93±0.10 and 1.47±0.14, all<0.05). The migration and invasion abilities of transfected Siha cells were not significantly changed (all>0.05).The expression of miR-let-7e-3p is down-regulated in cervical neoplasms, which is associated with cell cycle arrest and proliferation inhibition of cervical cancer cells.
