Effect of RAD18-siRNA on proliferation and chemotherapy sensitivity of human esophageal squamous cell carcinoma ECA-109 cells.
- Author:
Pengrong LOU
1
,
2
;
Xiaonan SUN
3
;
Jundong ZHOU
4
;
Shitao ZOU
5
Author Information
1. Department of Radio-Oncology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310020, China
2. Department of Radio-Oncology, Ningbo First Hospital, Ningbo 315010, China.
3. Department of Radio-Oncology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310020, China. sunxiaonan@hotmail.com.
4. Department of Radio-Oncology, Suzhou Municipal Hospital, Nanjing Medical University, Suzhou 215001, China. zhoujundong330@163.com.
5. Suzhou Cancer Center Core Laboratory, Suzhou Municipal Hospital, Nanjing Medical University, Suzhou 215001, China.
- Publication Type:Journal Article
- MeSH:
Adjuvants, Pharmaceutic;
pharmacology;
Carcinoma, Squamous Cell;
drug therapy;
physiopathology;
Cell Cycle;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cisplatin;
pharmacology;
Cyclin D1;
drug effects;
genetics;
DNA-Binding Proteins;
administration & dosage;
pharmacology;
Down-Regulation;
drug effects;
genetics;
Drug Resistance, Neoplasm;
drug effects;
Drug Screening Assays, Antitumor;
methods;
Drug Synergism;
Esophageal Neoplasms;
drug therapy;
physiopathology;
Fluorouracil;
pharmacology;
G1 Phase;
drug effects;
G2 Phase;
drug effects;
Humans;
Metaphase;
drug effects;
RNA, Small Interfering;
administration & dosage;
pharmacology;
Transfection;
Ubiquitin-Protein Ligases;
administration & dosage;
pharmacology
- From:
Journal of Zhejiang University. Medical sciences
2016;45(4):364-370
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of RAD18-siRNA on cell proliferation and chemotherapy sensitivity of esophageal squamous cell carcinoma (ESCC) ECA-109 cells.RAD18-siRNA was transfected into human ECA-109 cells by Lipofectamine 3000. Quantitative PCR and Western blot were performed to detect RAD18 and CyclinD1 expression; CCK-8 assay was used to determine cell proliferation and chemotherapy drug sensitivity; flow cytometry was used to determine cell cycle. Correlation between RAD18 and CyclinD1 mRNA expression was analyzed by Pearson's correlation.Compared with non-transfected cells, the expression of RAD18 in RAD18-siRNA group was significantly decreased (<0.05). The cell proliferation was inhibited (<0.05) and the cell number of G1 phase was increased, G2/M phase cells decreased (<0.05) in RAD18-siRNA group. After treatment with different concentrations of cisplatin or 5-FU, the survival rate of the two cell groups was reduced (all<0.05), and the IC50 of RAD18-siRNA group was significantly lower than that of non-transfected group (<0.05). The mRNA expression of RAD18 was positively correlated with CyclinD1 expression in ESCC tissues(=0.478,<0.01).Down-regulated expression of RAD18 can decrease the cell proliferation and increase chemo-sensitivity of ESCC cells, and CyclinD1 may participate in the process.
