Effect and its molecular mechanisms of curcumin on pulmonary artery smooth muscle cells in rat model with chronic obstructive pulmonary disease.
- Author:
Xiangang LIN
1
;
Yenong CHEN
1
;
Zhuqing LIU
1
Author Information
1. Acupuncture Clinical Traumatology Institute, Anhui University of Chinese Medicine, Hefei 230031, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
physiology;
Arterial Pressure;
drug effects;
physiology;
Curcumin;
pharmacology;
Hypertrophy, Right Ventricular;
pathology;
physiopathology;
Janus Kinase 2;
drug effects;
physiology;
Lung;
chemistry;
drug effects;
Male;
Myocytes, Smooth Muscle;
drug effects;
pathology;
Proliferating Cell Nuclear Antigen;
drug effects;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
drug effects;
metabolism;
Pulmonary Artery;
drug effects;
pathology;
Pulmonary Disease, Chronic Obstructive;
pathology;
physiopathology;
Rats;
Rats, Wistar;
STAT Transcription Factors;
Suppressor of Cytokine Signaling 3 Protein;
drug effects;
physiology;
Ventricular Pressure;
drug effects;
bcl-2-Associated X Protein;
drug effects;
metabolism
- From:
Journal of Zhejiang University. Medical sciences
2016;45(5):469-476
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects and the underlying molecular mechanisms of curcumin on pulmonary artery smooth muscle cells in rat model with chronic obstructive pulmonary disease (COPD).A total of 75 male Wistar rats were randomly divided into control group (group CN), model group (group M), low-dose curcumin group (group CL), medium-dose curcumin group (group CM) and high-dose curcumin group (group CH). HE staining was used to observe the morphology of pulmonary artery. Proliferating cell nuclear antigen (PCNA), apoptosis-related protein Bcl-2 and Bax were detected by immunohistochemical staining. TUNEL kit was used to analyze the effects of curcumin on apoptosis of smooth muscle cells, and the protein expressions of SOCS-3/JAK2/STAT pathway in lung tissues were determined by western blot.Right ventricular systolic pressure (RVSP) and right ventricular hypertrophy index (RVMI) in group M were significantly higher than those in group CN, group CH and group CM (all<0.05). HE staining and TUNEL kit test showed that the number of pulmonary artery smooth muscle cells had a significant increase in group M, while the pulmonary artery tube became thin, and the smooth muscle cells shrinked in group CM and group CH. Immunohistochemistry showed that PCNA and Bcl-2 in group M were significantly higher than those in group CN (all<0.05), while Bax expression was significantly lower than that in group CN (<0.05). PCNA in group CM and group CH were significantly lower than that in group M (all<0.05), while Bax expression was significantly higher than that in group M (<0.05). Western blot showed that SOCS-3 protein was significantly decreased in group M, while the p-JAK2, p-STAT1, p-STAT3 were significantly increased (all<0.05). Compared with group M, SOCS-3 protein in group CM and group CH were significantly increased (all<0.05), while the p-JAK2, p-STAT3 were significantly reduced (all<0.05).Curcumin could promote the apoptosis of smooth muscle cells in rats with COPD, and improve the mean pulmonary artery pressure and RVMI through stimulating SOCS-3/JAK2/STAT signaling pathway.
