Berberine regulates glycemialocal inhibition of intestinal dipeptidyl peptidase-Ⅳ.
- Author:
Jiesheng WANG
1
;
Guanhai DAI
2
;
Weijia LI
3
Author Information
1. Department of Cadre Health, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China.
2. Basic Laboratory, Institute of Traditional Chinese Medicine of Zhejiang Province, Hangzhou 310007, China.
3. Department of Endocrinology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China. liweijia0209@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Berberine;
pharmacokinetics;
pharmacology;
Blood Glucose;
drug effects;
Diabetes Mellitus, Experimental;
chemically induced;
drug therapy;
Dipeptidyl Peptidase 4;
analysis;
drug effects;
pharmacokinetics;
Dipeptidyl-Peptidase IV Inhibitors;
Dose-Response Relationship, Drug;
Glucagon-Like Peptide 1;
analysis;
blood;
Hypoglycemic Agents;
Insulin;
blood;
Intestines;
chemistry;
drug effects;
Male;
Rats;
Rats, Sprague-Dawley;
Sitagliptin Phosphate
- From:
Journal of Zhejiang University. Medical sciences
2016;45(5):486-492
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of berberine on glycemia regulation in rats with diabetes and the related mechanisms.Diabetic-like rat model was successfully induced by intraperitoneal injection of streptozotocin in 50 out of 60 male SD rats, which were then randomly divided into 5 groups with 10 rats in each:control group (received vehicle only), positive drug control group (sitagliptin 10 mg·kg·d), low-dose berberine group (30 mg·kg·d), moderate-dose berberine group (60 mg·kg·d), and high-dose berberine group (120 mg·kg·d). All animals were fed for 3 d, and fasting blood sampling was performed on day 3 of administration. Rats were given glucose (2 g/kg) by gavage 30 min after the last dose. Blood and intestinal samples were obtained 2 h after glucose loading. Fasting blood glucose (FBG) and 2-h postprandial plasma glucose (2h-PPG) were detected by using biochemical analyzer, and insulin, glucagon-like peptide-1 (GLP-1) and dipeptidyl peptidase-Ⅳ(DPP-Ⅳ) were measured by using ELISA kit.No significant difference in FBG and serum DPP-Ⅳ level were found between berberine groups and control group (all>0.05). Compared with control group, serum levels of GLP-1 and insulin were increased in high-and moderate-dose berberine groups, while 2h-PPG was decreased (all<0.05); GLP-1 levels in the intestinal samples were increased, while DPP-Ⅳ levels were decreased in all berberine groups (all<0.05).Short-term berberine administration can decrease 2h-PPG level in streptozotocin-induced diabetic rat model through local inhibition of intestinal DPP-Ⅳ. The efficacy of DPP-Ⅳ inhibitor may be associated with its intestinal pharmacokinetics.
