T cell receptor β-chain CDR3 spectratyping and cytomegalovirus activation in allogeneic hematopoietic stem cell transplant recipients.
- Author:
Zhihua WU
1
,
2
;
Min JING
3
;
Hanying LIANG
3
;
Rong YANG
3
;
Yaping HUANG
3
;
Xiaoming CHEN
3
;
Jianhua HU
3
;
Jun FAN
4
Author Information
1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
2. Clinical Laboratory, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China.
3. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
4. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. fanjun@zju.edu.cn.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Complementarity Determining Regions;
genetics;
immunology;
Cytomegalovirus;
genetics;
immunology;
Cytomegalovirus Infections;
genetics;
Genotype;
Hematopoietic Stem Cell Transplantation;
adverse effects;
Humans;
Lymphocyte Activation;
genetics;
Phosphoproteins;
Polymerase Chain Reaction;
Polymorphism, Genetic;
immunology;
Receptors, Antigen, T-Cell, alpha-beta;
genetics;
immunology;
Spleen;
T-Lymphocytes;
immunology;
virology;
Viral Matrix Proteins
- From:
Journal of Zhejiang University. Medical sciences
2016;45(5):515-521
- CountryChina
- Language:Chinese
-
Abstract:
To explore the association between T-cell receptor beta variable (TCR BV) complementarity determining region 3 (CDR3) spectratyping and CMV activation in the recipients of allogeneic hematopoietic stem cell transplantation (HSCT).Fluorescence quantitative PCR melting curve analysis was used to sequence 24 TCR BV families in 7 HSCT recipients and 3 healthy controls. CMV-pp65 antigenemia was measured by immunohistochemical staining. Plasma IgM specific for CMV was identified using ELISA. Relationship between TCR BV families and CMV activation was statistically analyzed.Twenty-four TCR BV families were expressed in 3 healthy controls, while TCR BV CDR3 sequencing results in 7 recipients turned out to be BV9, BV11, BV17, BV20 and so on. Amino acid sequence features were as follows:TCR BV9 contained "QVRGGTDTQ", TCR BV11 contained "VATDEQ" and "LGDEQ", TCR BV17 contained "IGQGNTEA", and TCR BV20 contained "VGLAANEQ". Five recipients suffered from pp65 antigenemia in 3 month after transplantation, and pp65-positive cells ranged from 2 to 15 per 5×10white blood cells. Three recipients were CMV-IgM positive. No significant differences were found in TCR BV families between pp65-positive recipients and pp65-negative recipients (all>0.05). But there was statistically significant difference in frequency of TCR BV11 between CMV-IgM negative recipients and CMV-IgM positive recipients (<0.05).T cell immune response was characterized by special TCR BV CDR3 spectratyping in HSCT recipients, and TCR BV11 expression may be associated with CMV activation.
