Identification of proteins interacting with the circadian clock protein PER1 in tumors using bacterial two-hybrid system technique.
- VernacularTitle:细菌双杂交筛选肿瘤细胞中与人生物钟蛋白PER1相互作用的蛋白
- Author:
Yu ZHANG
1
;
Youlin YAO
;
Siyuan JIANG
;
Yilu LU
;
Yunqiang LIU
;
Dachang TAO
;
Sizhong ZHANG
;
Yongxin MA
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Cell Line, Tumor; Escherichia coli; genetics; metabolism; Humans; Molecular Sequence Data; Neoplasms; genetics; metabolism; Period Circadian Proteins; genetics; metabolism; Protein Binding; Proteins; genetics; metabolism; Two-Hybrid System Techniques
- From: Chinese Journal of Medical Genetics 2015;32(2):192-197
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify protein-protein interaction partners of PER1 (period circadian protein homolog 1), key component of the molecular oscillation system of the circadian rhythm in tumors using bacterial two-hybrid system technique.
METHODSHuman cervical carcinoma cell Hela library was adopted. Recombinant bait plasmid pBT-PER1 and pTRG cDNA plasmid library were cotransformed into the two-hybrid system reporter strain cultured in a special selective medium. Target clones were screened. After isolating the positive clones, the target clones were sequenced and analyzed.
RESULTSFourteen protein coding genes were identified, 4 of which were found to contain whole coding regions of genes, which included optic atrophy 3 protein (OPA3) associated with mitochondrial dynamics and homo sapiens cutA divalent cation tolerance homolog of E. coli (CUTA) associated with copper metabolism. There were also cellular events related proteins and proteins which are involved in biochemical reaction and signal transduction-related proteins.
CONCLUSIONIdentification of potential interacting proteins with PER1 in tumors may provide us new insights into the functions of the circadian clock protein PER1 during tumorigenesis.
