Identification of 3 novel HLA-A alleles A*24:224, A*24:225 and A*24:257 by sequence-based typing.
- Author:
Chuanfu ZHU
1
;
Hongwei ZHANG
;
Yi ZHANG
;
Xiangmin NIE
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; ethnology; genetics; Base Sequence; China; ethnology; Genetics, Population; HLA-A24 Antigen; genetics; Histocompatibility Testing; Humans; Molecular Sequence Data; Polymorphism, Single Nucleotide; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2015;32(3):415-417
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo verify 3 novel HLA-A alleles A*24:224, A*24:225 and A*24:257 identified in Chinese Han individuals.
METHODSNo full matched results were obtained at HLA-A locus in HLA typing for China Marrow Donor Program (CMDP) using bi-allelic Sequence-Based Typing (SBT). The novel HLA alleles were identified with allele-specific amplification SBT.
RESULTSAll of the three probands had a novel nucleotide sequence at HLA-A locus. All of the 3 new sequences are most close to HLA-A*24:02:01:01 except for 1 or 2 nucleotide substitution in exon 2, which resulted in different changes in corresponding codons and encoded amino acids.
CONCLUSIONThree novel HLA-A alleles were confirmed and officially named as HLA-A*24:224, HLA-A*24:225 and HLA-A*24:257 under the GenBank accession numbers JQ899198, JQ924283 and HG003642 by the WHO Nomenclature Committee for Factors of the HLA System in November 2012 and November 2013, respectively.
