Expression and significance of miR-125a and Mcl-1 in intestinal tissue after massive small bowel resection in rat.
- Author:
Mengfei XIAN
1
;
Jinping MA
;
Sile CHEN
;
Jianwei LIN
;
Weiling HE
;
Dongjie YANG
;
Hui WU
;
Chuangqi CHEN
;
Shirong CAI
Author Information
- Publication Type:Journal Article
- MeSH: Anastomosis, Surgical; Animals; Disease Models, Animal; Intestine, Small; metabolism; surgery; Male; MicroRNAs; metabolism; Myeloid Cell Leukemia Sequence 1 Protein; metabolism; Rats; Rats, Sprague-Dawley; Short Bowel Syndrome; metabolism
- From: Chinese Journal of Gastrointestinal Surgery 2014;17(5):495-498
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and significance of miR-125a and anti-apoptotic protein Mcl-1 in intestinal tissue after massive small bowel resection in intestinal adaptation.
METHODSSprague-Dawley rats (54 male rats, 8-week old) were divided into 3 groups randomly, including two control groups. Rats in the experiment group were subjected to 70% massive small bowel resection. Rats in the resection group underwent simple intestinal resection and anastomosis. Rats in the control group underwent laparotomy alone. A 5 cm intestine approximately 1 cm distal to the anastomosis was harvested a week after operation. Expression of Mcl-1 was assessed by immunohistochemistry and real-time PCR was used to detect the expression of miR-125a in intestinal tissue.
RESULTSThe positive expression of Mcl-1 in the experiment group was 18.8%(3/16), significantly lower than that in the control group(76.5%, 13/17) and the resection group (83.33%, 15/18)(both P<0.01). The expression of miR-125a in the experiment group was 1.92, significantly higher than that in the control group (1.01) and the resection group (1.05)(both P<0.01).
CONCLUSIONmiR-125a and anti-apoptotic protein Mcl-1 may play an important role in intestinal adaptation process and they may regulate each other through a certain pathway.
