Quantitative analyses of intrahepatic HBV cccDNA and serum HBsAg in 54 patients with chronic hepatitis B.
- Author:
Wei-jie LI
1
;
Bo-an LI
;
Jing-min ZHAO
;
Jia-qi HAN
;
Yan LIU
;
Ling JIANG
;
Yuan-li MAO
;
Feng-min LU
;
Dong-ping XU
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Circular; analysis; DNA, Viral; analysis; Female; Hepatitis B Surface Antigens; blood; Hepatitis B virus; genetics; Hepatitis B, Chronic; blood; virology; Humans; Liver; virology; Male; Viral Load
- From: Chinese Journal of Hepatology 2011;19(11):815-817
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo quantitatively detect intrahepatic HBV covalently closed circular DNA (cccDNA) and serum HBsAg; and to analyze the relationship between the two parameters and with serum HBV DNA level.
METHODSIntrahepatic cccDNA (copies/cell) was quantitated by plasmid-safe ATP-dependent Danes (PSAD) digestion in combination with rolling circle amplification and gap-spanning selective real-time PCR assay using formalin fixed paraffin-embedded liver biopsy samples. HBsAg was measured by chemiluminescence's reagent manufactured by Abbott Company using sera sampled at time-point of liver biopsy.
RESULTSIntrahepatic cccDNA level was positively correlated with serum HBsAg level (r = 0.459, P < 0.001), but not correlated with serum HBV DNA level. Serum HBsAg level was positively correlated with serum HBV DNA level (r = 0.328, P = 0.015), and reversely correlated with HBV replicative efficiency defined as the ratio of serum HBV DNA to cccDNA (r = -0.373, P = 0.005).
CONCLUSIONIn patients with chronic hepatitis B, intrahepatic cccDNA level is correlated with serum HBsAg level. The two parameters combined with serum HBV DNA may comprehensively reflect HBV replication activity and help evaluation of antiviral therapeutic efficacy.
