E-cadherin expression and CDH1 promoter methylation in sporadic and hereditary gastric cancer.

Xiao-jun LI; Yang Zhao; Hong Ren

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E-cadherin expression and CDH1 promoter methylation in sporadic and hereditary gastric cancer.

Author: Xiao-Jun LI ¹ ; Yang ZHAO ; Hong REN
Author Information

1. Second Department of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.E-mail: szhaoy@126.com.
Publication Type:Journal Article
MeSH: Cadherins; genetics; metabolism; DNA Methylation; Humans; Promoter Regions, Genetic; Stomach Neoplasms; genetics; metabolism
From: Journal of Southern Medical University 2015;35(1):125-127
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo evaluate the role of E-cadherin (E-cad) and CDH1 gene encoding E-cad in the occurrence of sporadic or hereditary gastric cancer.
METHODSNineteen normal gastric mucosal issue specimens, 19 specimens of hereditary gastric cancer (diagnosed according to ICG-HGC criteria), and 19 specimens of sporadic gastric cancer examined for E-cad expression and CDH1 promoter methylation using immunohistochemistry and methylation-specific PCR (MSP).
RESULTSThe protein expression of E-cad were significantly reduced in both of the cancer tissues (P<0.001) compared with that in the normal gastric mucosal tissues, and showed no significant difference between the two cancers (P=0.84). CDH1 promoter hypermethylation was found in 10 out of the 19 hereditary gastric cancer tissues, a rate significantly higher than that in sporadic gastric cancer tissues (3/19, P<0.01).
CONCLUSIONCDH1 promoter hypermethylation is probably an important factor contributing to reduced E-cad expression in sporadic gastric cancer but not in hereditary gastric cancer.