Src kinase inhibitor PP2 protects rat astrocytes from hypoxia/reoxygenation injury in vitro.
- Author:
Yuchen GU
1
;
Xuhui TONG
;
Li YU
;
Hao JIAO
;
Binbin YU
;
Shuying DONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Astrocytes; pathology; Cell Hypoxia; Cells, Cultured; Flow Cytometry; Pyrimidines; pharmacology; Rats; src-Family Kinases; antagonists & inhibitors
- From: Journal of Southern Medical University 2015;35(2):239-243
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Src kinase inhibitor PP2 on hypoxia/reoxygenation (H/R) injury in rat astrocytes in vitro.
METHODSIn vitro cultured rat astrocytes were exposed to hypoxia for 8 h followed by reoxygenation for 24 h with or without pretreatment with PP2 (10 µmol/L) for 24 h before H/R injury. MTT assay and flow cytometry were used to detect the viability and apoptosis of the exposed astrocytes, respectively, and the protein expressions of Src, Bax, and Bcl-2 in the cells were determined using Western blotting.
RESULTSPP2 pretreatment significantly increased the viability and decreased the apoptosis rate of rat astrocytes exposed to H/R injury (P<0.01). Western blotting showed that H/R injury caused increased expression of Src kinase, which was lowered by PP2 pretreatment. The ratio of Bax/bcl-2 in the astrocytes increased after H/R injury, and was significantly decreased by PP2 pretreatment (P<0.01).
CONCLUSIONPP2 protects rat astrocytes from H/R injury possibly by inhibiting the expression of Src kinase and activating the anti-apoptotic mechanisms in the cells.
