Preparation and characterization of uricase in uricase-catalase liposomes prepared using borate buffer.
- Author:
Yunli ZHOU
1
;
Lin YANG
;
Zijun YAN
;
Xue DENG
;
Jingqing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Borates; Catalase; Liposomes; Nanoparticles; chemistry; Temperature; Urate Oxidase; chemistry
- From: Journal of Southern Medical University 2015;35(2):268-271
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo characterize the property of uricase loaded in uricase-catalase liposomes (BUCLPs) prepared using borate buffer.
METHODSBUCLPs were prepared using reverse-phase evaporation, and the physicochemical properties of uricase in the prepared BUCLPs were examined.
RESULTSThe optimal temperature of BUCLP and URI was 40 degrees celsius, their optimal pH values were 8.0 and 8.5, and their Michaelis-Menten constants were 14.207 µmol/L and 13.623 µmol/L, respectively. Fluorescence intensity of nanoliposome-loaded uricase-catalase that bound to FITC was higher than that of uricase-catalase binding directly with FITC; the fluorescence intensity of BUCLP was higher than that of free uricase-catalase at 280 nm.
CONCLUSIONUricase activity is enhanced after loading in uricase and catalase liposomes.
