Expression of transient receptor potential canonical 1 in ozone-induced inflammatory lung tissues in mice.
- Author:
Zhaodi FU
1
;
Lifen ZHOU
;
Jianrong HUANG
;
Shuyi GUO
;
Jiechun ZHANG
;
Yongbiao FANG
;
Xiaoai LIU
;
Qingzi CHNE
;
Jianhua LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bronchoalveolar Lavage Fluid; Disease Models, Animal; Gene Expression; Inflammation; pathology; Lung; metabolism; pathology; Mice; Ozone; adverse effects; Pneumonia; metabolism; pathology; RNA, Messenger; TRPC Cation Channels; metabolism
- From: Journal of Southern Medical University 2015;35(2):284-291
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of transient receptor potential canonical 1 (TRPC1) in a mouse model of ozone-induced lung inflammation and explore its role in lung inflammation.
METHODSIn a mouse model of lung inflammation established by ozone exposure, the expression of TRPC1 in the inflammatory lung tissues was detected by RT-PCR, Wstern blotting and immunohistochemistry.
RESULTSCompared to the control mice, the mice exposed to ozone showed significantly increased expression level of TRPC1 mRNA and protein in the inflammatory lung tissues (P<0.05). Immunohistochemistry showed increased TRPC1 protein expressions in the alveolar epithelial cells, bronchial epithelial cells, and inflammatory cells in the inflammatory lung tissues (P<0.05). The mRNA and protein expression levels of TRPC1 were positively correlated with the counts of white blood cells, macrophages, neutrophils and lymphocytes in the bronchoalveolar lavage fluid of the exposed mice (P<0.01).
CONCLUSIONTRPC1 may play a role in ozone-induced lung inflammation in mice.
