Expression of miR-223 in clear cell renal cell carcinoma and its significance.
- Author:
Shaoxi NIU
1
;
Xin MA
;
Yu ZHANG
;
Huijie GONG
;
Yu GAO
;
Xintao LI
;
Donglai SHEN
;
Lei WANG
;
Yuanxin YAO
;
Xu ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Renal Cell; metabolism; Cell Line, Tumor; Cell Movement; Cell Proliferation; Flow Cytometry; G1 Phase Cell Cycle Checkpoints; Humans; MicroRNAs; metabolism; Real-Time Polymerase Chain Reaction; Transfection; Up-Regulation
- From: Journal of Southern Medical University 2015;35(3):338-342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of miR-223 in clear cell renal cell carcinoma (ccRcc) and its clinical implications.
METHODSQuantitative real-time PCR was employed to detect the levels of miR- 223 expression in ccRcc, pair-matched adjacent normal tissues and different renal cancer cell lines. Transwell migration essay and wound healing essay were used to evaluate the invasion and migration of renal cancer 786-O cells transfected with miR-223 mimics. MTT essay was used to measure the cell proliferation, and the cell cycle changes following the transfection were analyzed with flow cytometry.
RESULTSCompared with the normal tissues, the cancer samples showed up-regulated miR-223 expression, which was associated with tumor size. In 786-O cell cultures, transfection with miR-223 mimics significantly enhanced cell migration (P<0.0001) and growth (P=0.006) and induced G1 cell cycle arrest.
CONCLUSIONmiR-223 promotes renal cancer cell migration and proliferation and may serve as a potential therapeutic target for ccRcc.
