Effect of anacardic acid, a Hsp90 inhibitor, on proliferation, invasion and migration of breast cancer MDA-MB-231 cells.
- Author:
Hongmei LI
1
;
Lijuan NIE
;
Qiang HUO
;
Surong ZHAO
;
Tao MA
;
Chengzhu WU
;
Hao LIU
Author Information
- Publication Type:Journal Article
- MeSH: Anacardic Acids; pharmacology; Breast Neoplasms; pathology; Cell Line, Tumor; drug effects; Cell Movement; Cell Proliferation; Down-Regulation; HSP90 Heat-Shock Proteins; antagonists & inhibitors; metabolism; Humans; Matrix Metalloproteinase 9; metabolism; Tissue Inhibitor of Metalloproteinase-1; metabolism
- From: Journal of Southern Medical University 2015;35(3):355-359
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of the Hsp90 inhibitor anacardic acid on cell proliferation, invasion and migration of breast cancer MDA-MB-231 cells.
METHODSThe inhibitory effect of anacardic acid on Hsp90 was assessed with in vitro ATPase inhibition assay and ATP-sepharose binding assay. MTT assay was used to detect the growth inhibition induced by anacardic acid in MDA-MB-231 cells. Transwell assays were used to evaluate MDA-MB-231 cell invasion and migration. Western blotting was performed to assess the effect of anacardic acid in triggering the degradation of MMP-9, TIMP-1, Hsp90, and Hsp70.
RESULTSAnacardic acid exhibited a modest activity of ATPase inhibition with an IC50 value of 82.5 µmol/L. Anacardic acid significantly suppressed the proliferation of MDA-MB-231 cells in a dose-dependent manner (IC50 value of 29.3 µmol/L). Treatment with 12.5, 25, and 50 µmol/L anacardic acid for 36 h caused inhibition of cell invasion by 23.6%, 56.6%, and 67.0% in MDA-MB-231 cells, respectively (P<0.05), and anacardic acid treatment for 24 h inhibited the cell migration by 30.0%, 45.5%, and 77.5%, respectively (P<0.05). Anacardic acid dose-dependently induced MMP-9 degradation, but did not obviously affect Hsp90 or Hsp70 expressions.
CONCLUSIONAnacardic acid can significantly inhibit the proliferation, invasion, and migration of MDA-MB-231 cells, the mechanism of which may involve the inhibition of Hsp90 ATPse activity and down-regulation of MMP-9 expression.
