Screening and identification of proteins related to gastric cancer metastasis with comparative proteomics.
- Author:
Yong LI
1
;
Lili WANG
;
Aiwen KANG
;
Liqiao FAN
;
Qun ZHAO
;
Bibo TAN
;
Yingjie HAO
;
Qingwei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Annexins; metabolism; Blotting, Western; Cell Line, Tumor; Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional; Heterogeneous-Nuclear Ribonucleoprotein Group A-B; metabolism; Humans; Kruppel-Like Transcription Factors; metabolism; Lymphatic Metastasis; Mice; Mice, Nude; Neoplasm Proteins; metabolism; Neoplasm Transplantation; Proteomics; RNA, Small Interfering; Stomach Neoplasms; pathology; Transfection
- From: Journal of Southern Medical University 2015;35(3):360-364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen and identify the proteins related with tumor metastasis of gastric cancer in a nude mouse model bearing orthotopic transplanted tumor.
METHODSZinc finger protein 139 (ZNF139)-specific siRNA was synthesized and transfected into gastric cancer cell line SGC7901, which was then screened by G418. ZNF139-siRNA-transfected cells, negative plasmid-transfected cells and untreated SGC7901 cells were orthotopically transplanted separately on the stomach wall of BALB/c nude mice. The primary tumors and metastatic lymph nodes were harvested to separate the proteins by 2-D fluorescence difference gel electrophoresis (2-D DIGE); after gel digestion, the differential proteins were subjected to liquid chromatography-mass spectrometry (LC-MS) for identification and their functions were analyzed. Western blotting was performed to verify the identified proteins.
RESULTSZNF139 expression was effectively inhibited in siRNA-transfected SGC7901 cells. ZNF139-siRNA-transfected cells showed obviously suppressed tumor growth with a lowered lymph node metastasis rate in nude mice compared with untreated cells and the negative control cells (P<0.05). Proteomic study with 2-D DIGE showed that fascin and hnRNPA2/B1 were down-regulated while ANXA1 was up-regulated in the primary tumors, and ANXA5 was down-regulated in the metastatic lymph nodes in ZNF139-siRNA-transfected group. Western blotting confirmed the results of proteomic analysis.
CONCLUSIONZNF139 gene may promote lymph node metastasis of gastric cancer by regulating fascin, hnRNPA2/B1, ANXA1, and ANXA5.