Screening of highly efficient small interfering RNAs against bone morphogenetic protein-7.
- Author:
Caixia LI
1
;
Peng BAI
;
Xian ZHAO
;
Yuan DONG
;
Xiaoming DAI
;
Yisong LI
;
Liu LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Morphogenetic Protein 7; genetics; metabolism; Cells, Cultured; Endothelial Cells; metabolism; RNA, Messenger; RNA, Small Interfering; Rats; Real-Time Polymerase Chain Reaction; Transfection
- From: Journal of Southern Medical University 2015;35(3):403-406
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo obtain highly efficient small interfering RNAs (siRNAs) against bone morphogenetic protein-7 (BMP-7) for future functional analysis of BMP-7 in bone homeostasis.
METHODSWe designed 4 independent siRNA sequences against rat BMP-7 and transfected them in rat umbilical vein endothelial cells. After 72 h, we examined BMP-7 mRNA and protein level in the transfected cells using real-time PCR and Western blotting, respectively.
RESULTSAll the 4 siRNAs effectively reduced BMP-7 expression in rat umbilical vein endothelial cells, and among them BMP-7-3 siRNA showed the highest efficiency.
CONCLUSIONWe have obtained an efficient siRNA to knockdown BMP-7 expression in vitro for further investigation of the role of BMP-7 in bone and cartilage formation.
