Expression of miR-200a in colorectal carcinoma cell lines and its effect on LoVo cells.
- Author:
Gongfa WU
1
;
Haiyan ZHAO
;
Nan HE
;
Huixia HAN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; Colorectal Neoplasms; metabolism; Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs; metabolism; Transfection
- From: Journal of Southern Medical University 2015;35(3):450-454
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect miR-200a expression in human colorectal carcinnoma (CRC) cell lines and explore the role of miR-200a in regulating the biological behavior of CRC cells.
METHODSReal-time quantitative RT-PCR (qRT-PCR) was used to detect miR-200a expression levels in 6 CRC cell lines (HCT116, HT29, LS174T, SW480, SW620 and LoVo). miR-200a mimics were transiently transfected into LoVo, and the changes in cell proliferation, apoptosis, migration, and cell-cell adhesion were assessed using CCK-8 assay, TUNEL assay, transwell migration assay, and homogenous adhesion experiment, respectively.
RESULTSThe expression of miR-200a was down-regulated in the 6 CRC cell lines, among which the highly metastatic LoVo cell line showed the lowest expression and the tumorigenic but non-metastatic CRC cell line HCT116 had the highest expression. Overexpression of miR-200a depressed cell proliferation and migration but promoted cell apoptosis and cell-cell adhesion in LoVo cells.
CONCLUSIONmiR-200a plays a role in regulating the invasiveness and metastasis of CRC, and overexpression of miR-200a causes a significant reduction of cell proliferation and migration and promotes apoptosis and cell-cell adhesion in LoVo cells.
