Inhibition of salicylate on potassium channels in rat inferior colliculus neurons.
- Author:
Yan-xing LIU
1
;
Xue-pei LI
;
Jun-xiu LIU
;
Ge-ming SHI
;
Hong LU
;
Chang-sheng MA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Delayed Rectifier Potassium Channels; drug effects; Inferior Colliculi; cytology; drug effects; Male; Neurons; drug effects; physiology; Patch-Clamp Techniques; Potassium Channels; drug effects; physiology; Rats; Rats, Wistar; Salicylates; pharmacology
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(11):835-839
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo understand what role of the transient outward potassium channels and the delayed rectifier potassium channels play in the mechanism of salicylate-induced tinnitus.
METHODSThe effects of salicylate on the transient outward potassium channels and the delayed rectifier potassium channels in freshly dissociated inferior colliculus neurons of rats were studied, using the whole-cell voltage clamp method.
RESULTSSalicylate blocked the transient outward potassium current (I(K(A and the delayed rectifier potassium current (I(K(DR in concentration-dependent manner (0.1-1 mmol/L). The IC50 values for the blocking action of salicylate on I(K(A)) and I(K(DR)) were 2.27 and 0.80 mmol/L, respectively. At a concentration of 1 mmol/L, salicylate did not shift the activation and inactivation curves of I(K(A)), but significantly shifted the activation and inactivation curves of I(K(DR)) negatively by approximately 11 mV and 24 mV.
CONCLUSIONSSalicylate inhibits both I(K(A)) and I(K(DR)) in rat inferior colliculus neurons but only significantly affects the activation and inactivation kinetics of I(K(DR)). Effects of I(K(A)) and I(K(DR)), especially I(K(DR)), by salicylate may play an important role in salicylate-induced tinnitus.
