Evaluating Home-made Disks for the Detection of the Aminoglycoside High-Level Resistance of Enterococci.
- Author:
Sung Ryul KIM
1
;
Joseph JEONG
;
Jeong Hwan SHIN
;
Hyung Hoi KIM
;
Seon Ho LEE
;
Chulhun L CHANG
;
Han Chul SON
Author Information
1. Department of Clinical Pathology, Pusan National University College of Medicine, Pusan, Korea. cchl@hyowon.pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Enterococcus;
Antimicrobial resistance;
High-level resistance;
Gentamicin;
Streptomycin
- MeSH:
Aminoglycosides;
Diffusion;
Enterococcus;
Gentamicins;
Streptomycin
- From:Korean Journal of Clinical Pathology
2000;20(4):379-383
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: High-level gentamicin and streptomycin disks are not easily available, despite their critical role in detection of high-level resistance to aminoglycosides in enterococci. Therefore, the possibility of applicating home-made disks to test high-level resistance of enterococci to aminoglycosides was evaluated. METHODS: The disk diffusion method using home-made disks was compared with minimal inhibitory concentrations(MIC) in 53 clinical isolates of enterococci, and, the stability of the disks were also evaluated by disk diffusion testing, biweekly, for 14 weeks. RESULTS: The high-level resistance rates to gentamicin(GM) and streptomycin(SM) were 60% and 43%, respectively. Thirty eight % of the enterococci were highly resistant in both GM and SM. The results of the disk diffusion method were consistent with the MIC until 10 weeks after production of the disks. After 12 weeks, the inhibition zones of GM- or SM-susceptible strains decreased by 2.9-3.9 mm, and the discrepancy rates were 5-24% between the results of the MIC and disk diffusion method. The storage temperature of -20degrees C versus -70degrees C showed no difference in the inhibition zone. CONCLUSION: It has been demonstrated that home-made high-level GM and SM disks are stable at -20degrees C for 10 weeks, and the results of disk diffusion method on the disks show they are applicable for the test of susceptibility of aminoglycosides to enterococci.
