Gossypol decreases the expression of connexin 43 in Sertoli cells.
- Author:
De-rong ZHOU
1
;
Jun-hong ZHENG
;
Qing-tao YANG
;
Bing-sen LIN
;
You-bin PENG
;
Qiong GAO
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Connexin 43; metabolism; Gossypol; toxicity; Humans; Male; Sertoli Cells; drug effects; metabolism
- From: National Journal of Andrology 2011;17(11):993-997
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of gossypol on the expression of connexin 43 (CX43) in Sertoli cells.
METHODSTM4 Sertoli cells were cultured and treated with gossypol at the concentrations of 1.25, 2.5, 5 and 10 micromol/L for 6, 12, 24 and 48 hours. The cytotoxicity of gossypol was assessed by CCK-8 assay, and the expression of CX43 in the normal TM4 Sertoli cells and in those treated with different concentrations of gossypol for different times was detected by RT-PCR and immunofluorescence analysis.
RESULTSSemiquantitative RT-PCR and immunofluorescence analysis showed the expression of CX43 in the normal TM4 cells. At 24 hours of exposure to gossypol, the expression began to decrease gradually with the prolonging of time and the increasing concentration of gossypol (P < 0.05).
CONCLUSIONGossypol reduces the expression of CX43 in TM4 Sertoli cells, which might underlie the mechanism of its antifertility action.
