Fasudil improves erectile function of hypertensive rats.
- Author:
Zhi-peng MA
1
;
Rui JIANG
;
Yong CHENG
;
Hai-fan YANG
;
Feng CHEN
Author Information
- Publication Type:Journal Article
- MeSH: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; analogs & derivatives; pharmacology; therapeutic use; Animals; Hypertension; physiopathology; Male; Penile Erection; drug effects; Rats; Rats, Sprague-Dawley; Vasodilator Agents; pharmacology; therapeutic use
- From: National Journal of Andrology 2012;18(1):11-15
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of fasudil, an inhibitor of Rho kinase, on the erectile function of hypertensive rats and its action mechanism.
METHODSTwenty 12-week-old healthy male Sprague-Dawley rats were randomly divided into groups A (control), B (hypertension) and C (fasudil treatment). After establishment of the hypertension model, group C received intraperitoneal injection of fasudil at 30 mg/(kg x d), while A and B normal saline only. At 10 weeks after surgery, we measured the corpus cavernosum pressure/mean carotid arterial pressure (ICPmax / MAP), and the expression levels of ROCK1 and ROCK2 proteins in the corpus cavernosum of the rats by Western-blot.
RESULTSThe systolic blood pressure (mmHg) and the expressions of ROCK1 and ROCK2 proteins were significantly increased in group B (190.39 +/- 5.07, 0.048 +/- 0.002 and 0.143 +/- 0.011) as compared with A (124.81 +/- 4.01, 0.036 +/- 0.001 and 0.101 +/- 0.011) (P<0.05), but markedly decreased in group C (182.03 +/- 4.32, 0.044 +/- 0.001 and 0.126 +/- 0.007) in comparison with B (P<0.05). ICPmax /MAP was significantly lower in group B (36.82 +/- 5.47) than in A (59.99 +/- 5.69) (P<0.05), but remarkably higher in group C (51.1 +/- 5.63) than in B (P<0.05).
CONCLUSIONFasudil can improve erectile function in hypertensive rats by inhibiting the expression of RhoA / Rho kinase signaling and its possible attenuating effect on hypertension.
