Expression of macrophage inflammatory protein-1alpha in EPS and its significance.
- Author:
Wei ZOU
1
;
Wu-jiang LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Body Fluids; metabolism; Case-Control Studies; Chemokine CCL3; metabolism; Chronic Disease; Humans; Male; Middle Aged; Prostate; secretion; Prostatitis; metabolism; Young Adult
- From: National Journal of Andrology 2012;18(1):63-66
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the mRNA and protein expressions of MIP-1alpha in EPS and determine their significance in the sub-typing of chronic prostatitis.
METHODSWe collected samples of expressed prostatic secretion (EPS) from 50 cases of chronic prostatitis, including 16 cases of chronic bacterial prostatitis (CBP), 23 cases of chronic nonbacterial prostatitis/chronic pelvic pain syndrome (CPPS) (11 CPPS IIIA, 12 CPPS IIIB), and 11 cases of type-IV asymptomatic inflammatory prostatitis (AIP). Another 15 healthy volunteers were included as normal controls. The mRNA and protein levels of MIP-1alpha in EPS were determined by RT-PCR and ELISA, respectively, followed by statistical analysis with SPSS 15.0.
RESULTSThe mRNA expression of MIP-1alpha was markedly higher in the CPPS IIIA and CPPS IIIB groups than in the others (P<0.05). The protein level of MIP-1alpha was (1174.3 +/- 89.2) pg/ml in CPPS IIIA and (842.3 +/- 76.2) pg/ml in CPPS IIIB, significantly higher than (198.0 +/- 37.8) pg/ml in the control, (347.0 +/- 61.6) pg/ml in CBP and (292.0 +/- 56.4) pg/ml in type-IV AIP (P<0.05).
CONCLUSIONDetermination of mRNA and protein levels of MIP-1alpha in EPS may help the sub-typing and diagnosis of chronic prostatitis.
